Technician Position in Molecular Microbiology

vor 2 Wochen

Wien, Österreich Max Perutz Labs Vienna Vollzeit

We are looking for a lab technician to work on an exciting FWF-funded project in basic research, which addresses novel regulatory mechanisms in the cell envelope stress response in the non-pathogenic model bacterium

The work will involve diverse methods including cultivation of bacteria, genetic engineering, protein purification, techniques for detection of RNA and protein (e.g. Northern/Western blotting), methods addressing interaction of biomolecules (pull-down, two-hybrid system) and others. Experience in working with bacteria and prior knowledge in Microbiology are highly appreciated but not a prerequisite to fill the position. As we are located at an international institute, good English skills are required.

**About the project**

Infection diseases caused by viruses and bacteria represent major threats for human health, livestock and agriculture. Gram-negative bacteria of the family _Enterobacteriaceae_ including pathogenic _E. coli_ strains are of particular concern as resistances against currently used antibiotics are rapidly emerging, demanding for novel therapies. These bacteria are protected by a cell wall sandwiched between an inner and an outer membrane, which forms an effective barrier limiting the efficacy of several antibiotics.

The cell envelope is crucial in sensing the external environment, activating intracellular signaling pathways through which the bacteria adapt to changing conditions. When they are exposed to antibiotics or other stresses that target the envelope, they activate multiple stress response systems to protect themselves. In our projects, we want to understand how these regulatory networks respond to envelope perturbation and achieve protection and repair.

**About the Görke lab**

Our laboratory is located at the Max Perutz labs - a joint venture Institute for Molecular Biology of the University of Vienna and the Medical University of Vienna on the Vienna BioCenter Campus. The laboratory is fully equipped and the campus offers access to multiple research facilities. Additional information on the Görke lab.

**What we offer**

**Application

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