Scripps Staff Scientist Martins Lab

Scripps Staff Scientist Classification Minimum Requirements : Ph.D. and / or MD in an appropriate scientific discipline required. A minimum of 2 years postdoctoral experience. Job Description The Martins lab at The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology is looking for a Staff Scientist. Our research program is focused on developing a one-shot functional cure for HIV / AIDS. To that end we are leveraging the latest advances in gene therapy to engineer long‑lived host cells to continuously produce HIV‑1 IgG therapeutics in vivo at levels sufficient to prevent viral replication in the absence of antiretroviral therapy (ART). Using nonhuman primates as a preclinical model of HIV / AIDS we have shown that a single dose of adeno‑associated virus (AAV) vectors at birth can result in stable expression of a HIV‑1 broadly neutralizing antibody (bNAb) in plasma for years without redosing. The systemic HIV‑1 immunity achieved by this strategy could block acquisition of simian‑HIV infection (SHIV) in mucosal challenge models that mimic HIV transmission through breastfeeding and sexual intercourse. Additionally ongoing studies in our group show that AAV‑vectored delivery of HIV‑1 IgG biologics can also prevent virus rebound in SHIV‑infected infant and adult rhesus macaques following ART interruption. Despite the promise of these results AAV‑expressed bNAbs often induce host immune responses that restrict bNAb expression and lead to treatment failure. Although these anti‑drug antibody (ADA) responses are rarely observed in newborn macaques a large fraction of AAV / bNAb‑treated adult monkeys (and humans too) develop immune responses that interfere with the half‑life or biological activity of the delivered molecules. Thus a main focus of our lab is to understand why gene therapy with AAV / bNAb vectors induces ADAs in some animals but not in others in hopes devising strategies for suppressing these unwanted host immune responses. Responsibilities Project Management (meetings bookkeeping purchasing of reagents and supplies etc.) Grant / Manuscript writing Cloning cell culture ELISAs protein expression and purification and data analysis and interpretation Expected Salary : Commensurate with experience. Required Qualifications Ph.D. in immunology virology or a related discipline. Evidence of productivity from publications in high‑quality journals originating from Ph.D. or postdoctoral work. Manuscripts that are finally accepted for publication in high‑quality journals will also be considered as evidence of productivity. Significant expertise in the design and production of gene therapy vectors. Verbal and written communication skills in English with good organizational and analytical abilities. Strong managerial and organizational skills. Preferred Immunological techniques including flow cytometry ELISA immunofluorescence RNA / DNA scope. Molecular biology assays like qPCR and advanced cloning. Preclinical models of HIV / AIDS. Next‑generation sequencing and bioinformatics. Special Instructions to Applicants The University of Florida is a member of the State University System of Florida and an Equal Opportunity Employer. All qualified applicants will receive consideration for employment based on merit. If an accommodation due to a disability is needed to apply for this position please call or the Florida Relay System at (TDD) or visit Accessibility at UF. In order to be considered you must upload your cover letter and resume. Please provide names and email addresses of three to five references as part of the application process. When ready the hiring department will contact the listed references via email requesting their reference letters to be uploaded directly to the application website. This position is open until filled. Application review will begin September . This is a time-limited position. Health Assessment Required Yes. Required Experience Staff IC Key Skills Laboratory Experience,Bioinformatics,Biochemistry,Utility Locating,Assays,Research Experience,Next Generation Sequencing,Sensors,Signal Processing,Matlab,Research & Development,Molecular Biology Employment Type Full-Time Experience years Vacancy 1 #J-18808-Ljbffr