Postgraduate Research Associate

vor 2 Wochen

Wien, Österreich BOKU - Universität für Bodenkultur Wien Vollzeit

**_University of Natural Resources and Life Sciences Vienna - responsibility for Humans _**
**_and Nature. We work for the sustainable use and protection of natural resources.
- **

The Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology is currently
seeking a

**Postgraduate Research Associate**

(Reference code: 229)

Extent of employment: 30 hours per week
Duration of employment: 1st of March 2024, limited to 28th of February 2027

Workplace: 1190 Vienna, Muthgasse 18

Gross monthly salary and pay grade in terms of collective agreement for university staff (payable 14
times per year): B1, € 2.458,00

**Responsibilities**

Perform PhD thesis is desired

events, workshops and retreats
Carry out research project “More than just letters - the impact of protein sequence variation on

detailed project description)

Mutants of model enzymes, antibody fragments and non-antibody binder scaffold will be
expressed in yeast (soluble and/or yeast surface display YSD) and analysed regarding their
secretion yields, stress responses and biophysical properties

For one model protein it will be validated that expressibility is host species-independent by
expressing selected protein sequence variants in the yeast Komagataella phaffii (Pichia
pastoris) as well as in mammalian cells (human T-cells)

Additional mutations will be introduced into the model proteins through random mutagenesis
or targeted approaches

High-throughput correlation of cell stress to protein sequence variations by expressing
different sequence variants as surface displayed-protein libraries in yeast cells that contain
fluorescent biosensors for folding
- and secretion-related cellular responses and subsequent
analysis by flow cytometry

Selected variants will be analyzed in order to differentiate between mutations affecting
folding/secretion and activity/binding properties, as well as the more in-depth cellular response
to their expression

**Required skills and qualifications**

Diploma degree in Biotechnology, Molecular Biology, Biochemistry, Chemistry or closely

Language skills: English, German

**Desirable skills and qualifications**

Practical experience in molecular biology or biochemistry methods
Interest in microbial expression systems
Interest in computational analysis
Interest in working on an interdisciplinary topic
Confidence in communicating and presenting scientific work to colleagues and to public
An organized approach with strong attention to detail and good communication skills in

German and/or English with ability to work well in teams
Practical experience in one or more of the above mentioned protein characterization and

analysis methods desired

Applications can be submitted until: 8th of December 2023

University of Natural Resources and Life Sciences Vienna seeks to increase the number of its female
faculty and staff members. Therefore qualified women are strongly encouraged to apply. In case of equal

People with disabilities and appropriate qualifications are specifically encouraged to apply.

Copies of degree certificates
Copy of your passport

to Personnel Management, University of Natural Resources and Life Sciences, Peter-Jordan-Straße 70,

We regret that we cannot reimburse applicants travel and lodging expenses incurred as part of the
selection and hiring process.

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