Laboratory Technician in Vascular Heterogeneity
vor 2 Wochen
The laboratory of Laura de Rooij at CeMM, the Research Center for Molecular Medicine of the Austrian Academy of Sciences in Vienna, is seeking a highly motivated Laboratory Technician to join an international team working at the intersection of vascular and endothelial cell diversity, single-cell omics, and human aging/pathology. The lab is specifically interested in investigating the transcriptomic make-up and function of endothelial cells across human organs and tissues, using a combination of cutting-edge omics technologies, clinical samples, and in vitro model systems.
We are looking for a Laboratory Technician with a strong background in vascular biology, cell and molecular biology, or related fields. You will be responsible for lab organization and management, and you will contribute with your technical skills and research experience to the success of the research group. In addition, you will be a part of individual as well as group meetings to deepen the understanding of the ongoing scientific directions in the group. We provide the opportunity to be part of cutting-edge research projects in a highly supportive environment, with a time commitment of 18-24 months and with the potential for co-authorship in a high-impact publication. The preferred start date for this position is April or May.
Your profile- Degree in life sciences (BSc, MSc, Mag, DI) or equivalent training (e.g., HBLVA Rosensteingasse)
- Qualified laboratory experience in molecular and cellular biology and/or angiogenesis assays
- Experience in (endothelial) cell culture techniques is essential
- Familiarity with 3D cell culture (e.g. vessel/organ-on-chip), and/or human induced pluripotent stem cells is highly desirable
- Experience with human sample handling, multiplexed histology, and/or single-cell RNA-sequencing is a plus
- Excellent written and oral communication skills in German and English, excellent organizational skills
- High accuracy and reliability, able to work seamlessly in a dynamic and young team
- Laboratory management and organization, ordering of laboratory consumables
- Molecular biology, such as preparation of single cell RNA-sequencing libraries
- Cell culture, using 2D, 3D and/or microfluidics-based assays
- Cell isolation and sorting of primary human (endothelial) cells
- Functional vascular assays, such as migration or spheroid sprouting
- Immunofluorescence staining of cell cultures and/or human biopsy material
- Phenotyping of endothelial cells using multicolour flow cytometry
The goal of the Laura de Rooij laboratory is to study the gene expression landscape of the vascular endothelium, one cell at a time. Utilizing advanced single-cell RNA sequencing technologies, our lab is dedicated to mapping the diverse landscape of endothelial cell heterogeneity across various organs, tissues, pathological conditions, and throughout the aging process. Through this comprehensive approach, we seek to uncover novel genes and molecular pathways linked to vascular aging and disease.
The Principal InvestigatorLaura de Rooij studied biomedical sciences at the University of Amsterdam (The Netherlands). She then joined the Stem Cell and Cancer Research Institute at McMaster University in Hamilton (Canada), where she studied the role of RNA-binding proteins in leukemic stem cells via an in vivo two-step CRISPR-Cas9-mediated screening approach. For her postdoctoral studies, she returned to Europe to work under the mentorship of Professor Carmeliet in the lab of Angiogenesis and Vascular Metabolism at VIB-KU Leuven (Belgium). There, she led and contributed to numerous single-cell transcriptome atlases of endothelial cells, generated from a diverse range of tissues, preclinical models, and clinical patient material in health and disease. Laura de Rooij joined CeMM as principal investigator in September 2022. Since then, her lab focuses on deciphering the transcriptomic landscape and the role of endothelial cells in human health, aging, and pathology.
About the InstituteThe mission of CeMM, the Research Center for Molecular Medicine of the Austrian Academy of Sciences is to achieve maximum scientific innovation in molecular medicine to improve healthcare. At CeMM, an international and creative team of scientists and medical doctors pursues free-minded basic life science research in a large and vibrant hospital environment of outstanding medical tradition and practice. CeMM's research is based on post-genomic technologies and focuses on societally important diseases, such as immune disorders and infections, cancer and metabolic disorders. CeMM operates in a unique mode of super-cooperation, connecting biology with medicine, experiments with computation, discovery with translation, and science with society and the arts.
CeMM is a proud recipient of the HR Excellence in Research Award (HRS4R). This award demonstrates that CeMM cares about the well-being of its staff, that the recruitment process is open, fair, and transparent, and that CeMM provides professional appraisals and career development procedures. CeMM employs more than 300 members, including auxiliary groups, from 47 nationalities. The working language is English. The Institute promotes equal opportunities and is home to a mix of different talents, backgrounds, and competencies.
- Work within an experienced, interdisciplinary, and international team at one of Austria's leading research institutes
- Ample opportunities to contribute and gain experience in a key area of biomedical research and precision medicine
- An inspiring workplace with an international setting, strong team spirit, and an excellent work climate
- A wide range of social, cultural, and sports activities organized by the institute
- Excellent employee benefits including full insurance coverage (health, accident, retirement), health care services, subsidized cafeteria
- Support for relocating to Vienna is provided (relocation reimbursement, visa support, etc.)
Please apply online (https://cemm.onlyfy.jobs/job/fu04npu5) with cover letter, CV and contact details of 2 referees. Applications will be reviewed on a rolling basis. Any application received by 20 March 2025 will be considered.
Additional informationCity Vienna
Position type Full-time employee
Start of work 01.04.2025
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