Master’s Position in Molecular Cell Biology: Dna

vor 3 Wochen


Wien, Österreich Max Perutz Labs Vienna Vollzeit

The main goal of this MSc project is to analyze replication factories by high-resolution microscopy and uncover how the interaction of PARG with PCNA contributes to the regulation of DNA replication and replication stress response.

**About the research project**

DNA replication is a highly dynamic process that needs to be tightly coordinated. The DNA sliding clamp PCNA acts as a scaffold for the recruitment of DNA replication and DNA repair factors. PCNA forms characteristic foci in S-phase cells marking replication factories. Replication factories may act as biomolecular condensates to maintain adequate concentrations of replication factors.

PCNA recruits PARG to replication factories, which degrades poly(ADP-ribose) (PAR) and is required for recovery from prolonged replication stress. PARG inhibitors are clinically relevant for synthetic lethality approaches in cancer patients that are deficient in homologous recombination.

We found that PARG strongly associates with PCNA through a PCNA-interacting protein motif (PIP-box) within its disordered regulatory region. This interaction triggers condensate formation of PARG and PCNA in vitro and regulates their clustering within replication foci in cells.

**About the position**

The project's main goal is to analyze replication factories by high-resolution microscopy and uncover how the interaction of PARG with PCNA contributes to the regulation of DNA replication and replication stress response. During the project you will gain expertise in the following techniques: cell culture, immunofluorescence, confocal microscopy, Airy scan high-resolution imaging, image analysis.

The MSc thesis will be carried out in the Slade group at Max Perutz Labs. The start date is preferably June 2024 for the duration of 10-12 months. The student will receive a stipend of about €450 per month.

**Application and contact**

More details on the research topics of the Slade lab.



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